Platelet-activating factor downregulates the expression of liver X receptor-α and its target genes in human neutrophils

Liver X receptors (LXRs) are ligand-activated members of the nuclear receptor superfamily that regulate the expression of genes involved in lipid metabolism and inflammation, although their role in inflammation and immunity is less well known. It has been reported that oxysterols/LXRs may act as anti-inflammatory molecules, although opposite actions have also been reported. In this study, we investigated the effect of platelet-activating factor (PAF), a proinflammatory molecule, on LXRα signalling in human neutrophils. We found that PAF exerted an inhibitory effect on mRNA expression of TO901317-induced LXRα, ATP-binding cassette transporter A1, ATP-binding cassette transporter G1, and sterol response element binding protein 1c. This negative action was mediated by the PAF receptor, and was dependent on the release of reactive oxygen species elicited by PAF, as it was enhanced by pro-oxidant treatment and reversed by antioxidants. Current data also support the idea that PAF induces phosphorylation of the LXRα molecule in an extracellular signal-regulated kinase 1/2-mediated fashion. These results suggest that a possible mechanism by which PAF exerts its proinflammatory effect is through the downregulation of LXRα and its related genes, which supports the notion that LXRα ligands exert a modulatory role in the neutrophil-mediated inflammatory response.

Validación local de la temperatura superficial del mar del sensor MODIS en aguas someras del Mediterráneo occidental

La temperatura superficial del mar (SST) estimada a partir de los productos 11 μm diurnos y nocturnos y 4 μm nocturnos del sensor MODIS (Moderate Resolution Imaging Spectroradiometer) a bordo de la plataforma Aqua, han sido comparados con datos medidos in situ a tres profundidades diferentes (15, 50 y 100 cm) en una zona costera del Mediterráneo Occidental. Esta comparación ha permitido analizar la incertidumbre que existe en la estimación de este parámetro en aguas someras y próximas a la costa mediante imágenes de satélite de baja resolución espacial. Los resultados obtenidos demuestran que el producto diurno SST_11 μm, obtiene los estadísticos RMSE (error cuadrático medio) y r2 (coeficiente de correlación de Pearson) más ajustados con valores de 1°C y 0,96, respectivamente, para la profundidad 50 cm.

Oleic acid modulates mRNA expression of liver X receptor (LXR) and its target genes ABCA1 and SREBP1c in human neutrophils

Purpose: Regulation of liver X receptors (LXRs) is essential for cholesterol homeostasis and inflammation. The present study was conducted to determine whether oleic acid (OA) could regulate mRNA expression of LXRα and LXRα-regulated genes and to assess the potential promotion of oxidative stress by OA in neutrophils. Methods: Human neutrophils were treated with OA at different doses and LXR target gene expression, oxidative stress production, lipid efflux and inflammation state were analyzed. Results: We describe that mRNA synthesis of both LXRα and ABCA1 (a reverse cholesterol transporter) was induced by OA in human neutrophils. This fatty acid enhanced the effects of LXR ligands on ABCA1 and LXR expression, but it decreased the mRNA levels of sterol regulatory element-binding protein 1c (a transcription factor that regulates the synthesis of triglycerides). Although OA elicited a slight oxidative stress in the short term (15–30 min) in neutrophils, it is unlikely that this is relevant for the modulation of transcription in our experimental conditions, which involve longer incubation time (i.e., 6 h). Of physiological importance is our finding that OA depresses intracellular lipid levels and that markers of inflammation, such as ERK1/2 and p38 mitogen-activated protein kinase phosphorylation, were decreased by OA treatment. In addition, 200 μM OA reduced the migration of human neutrophils, another marker of the inflammatory state. However, OA did not affect lipid peroxidation induced by pro-oxidant agents. Conclusions: This work presents for the first time evidence that human neutrophils are highly sensitive to OA and provides novel data in support of a protective role of this monounsaturated acid against the activation of neutrophils during inflammation.

Efectos del cambio climático y usos del suelo sobre los recursos hídricos de la cuenca del río Tordera (Barcelona, España)

Se presentan los efectos del cambio global en la cuenca del río Tordera (España) para el periodo 2000-2050, escenarios climáticos A2 (medio-alto) definidos por el Panel Intergubernamental del Cambio Climático (IPCC, 200) y escenarios socioeconómicos (cambios previstos en la cuenca) denominados estable y tendencial. Los efectos sobre los recursos hídricos se han analizado de forma conjunta superficial-subterránea mediante una metodológica de tipo acoplado. Para establecer los impactos futuros sobre los recursos hídricos se ha seleccionado el Modelo de Circulación Global ECHAM5 (Max Planck Institute). Los resultados obtenidos indican una disminución de la precipitación del 11.3% y un aumento de la temperatura de 1ºC, respecto a los valores históricos de la zona. De acuerdo a la proyección futura (2050) sobre cambios en los recursos hídricos, la escorrentía superficial obtenida mediante simulación con el código HEC-HMS 3.4 experimenta una reducción del 31.8% respecto al valor histórico y la recarga natural, estimada mediante VISUAL-Balan, se reduce en un 11.7%. El balance en el acuífero deltaico simulado mediante MODFLOW 2009.1 Pro muestra igualmente una disminución de los parámetros del balance. Los cambios del uso del suelo previstos de acuerdo a la legislación vigente (escenarios socioeconómicos) no conducen a la generación de un impacto apreciable en los recursos hídricos; según los escenarios definidos la variación de precipitación y temperatura son los parámetros fundamentales del cambio previsto.

The Early Miocene “Bisciaro volcaniclastic event” (northern Apennines, Italy): a key study for the geodynamic evolution of the central-western Mediterranean

The Early Miocene Bisciaro Fm., a marly limestone succession cropping out widely in the Umbria–Romagna–Marche Apennines, is characterized by a high amount of volcaniclastic content, characterizing this unit as a peculiar event of the Adria Plate margin. Because of this volcaniclastic event, also recognizable in different sectors of the central-western Mediterranean chains, this formation is proposed as a “marker” for the geodynamic evolution of the area. In the Bisciaro Fm., the volcaniclastic supply starts with the “Raffaello” bed (Earliest Aquitanian) that marks the base of the formation and ends in the lower portion of the Schlier Fm. (Late Burdigalian–Langhian p.p.). Forty-one studied successions allowed the recognition of three main petrofacies: (1) Pyroclastic Deposits (volcanic materials more than 90 %) including the sub-petrofacies 1A, Vitroclastic/crystallo-vitroclastic tuffs; 1B, Bentonitic deposits; and 1C, Ocraceous and blackish layers; (2) Resedimented Syn-Eruptive Volcanogenic Deposits (volcanic material 30–90 %) including the sub-petrofacies 2A, High-density volcanogenic turbidites; 2B, Low-density volcanogenic turbidites; 2C, Crystal-rich volcanogenic deposits; and 2D, Glauconitic-rich volcaniclastites; (3) Mixing of Volcaniclastic Sediments with Marine Deposits (volcanic material 5–30 %, mixed with marine sediments: marls, calcareous marls, and marly limestones). Coeval volcaniclastic deposits recognizable in different tectonic units of the Apennines, Maghrebian, and Betic Chains show petrofacies and chemical–geochemical features related to a similar calc-alkaline magmatism. The characterization of this event led to the hypothesis of a co-genetic relationship between volcanic activity centres (primary volcanic systems) and depositional basins (depositional processes) in the Early Miocene palaeogeographic and palaeotectonic evolution of the central-western Mediterranean region. The results support the proposal of a geodynamic model of this area that considers previously proposed interpretations.